绒毛蛋白, 肠上皮细胞, 组织特异性 Cre 小鼠, 重组酶, 原位表达

Objective To investigate the expression of Cre recombinase in a kind of new intestinal epithelial-specific Cre transgenic mouse. Methods

The expression of Cre recombinase in Vil1-Cre mouse could be investigated by detecting the expression of the reporter gene YFP, of which the Rosa26-YFP fluorescence reporter gene expression could be induced by Cre recombinase. Results In adult, Vil1-Cre mouse expressed Cre recombinase in the whole intestinal epithelium, including duodenum, jejunum,ileum, and colon. Meanwhile, a few scattered cells expressing Cre recombinase were observed in other tissues such as the kidney, pancreas, testis and ovary. During embryonic development, Cre recombinase is already expressed in the midgut at embryonic day 10. 5 ( E10. 5 ) , just one day later than the expression of endogenous villin protein at E9. 5. Besides, there is also a certain expression of Cre recombinase in the septum transversum, genital ridge and mesonephric tubule at E11. 5 and E12. 5.Conclusion The Vil1-Cre mouse showed a perfect expression pattern of Cre recombinase throughout the intestinal epithelium with high specificity and activity, which can be used to construct an intestinal epithelial-specific gene modified mouse model for related study.

villin, intestinal epithelial cell, tissue-specific Cre mouse, recombinant enzyme, in situ expression